Figure 5.

Photomicrographs of histology from a representative donor at week 5 and quantification of ARS staining for all 3 donors. (A, C): Photomicrographs of Safranin O (Saf O)/Fast Green (A) and ARS (C) histology of week 5 aggregates from donor B. (B, D): Higher magnification images of Saf O/Fast Green (B) and ARS (D) staining of same region in each group as indicated by black rectangles in (A) and (C), respectively. Scale bars = 500 μm (black), 100 μm (white). All images in each row are at the same scale. (E): Quantification of average ARS staining in the periphery and core of week 5 aggregates for all three donors. ▵, significantly different from group 3; ●, significantly different from group 6. Abbreviations: ARS, alizarin red S; BMP, bone morphogenetic protein; exo., exogenous; GAG, glycosaminoglycan; GM, gelatin microparticle; MCM, mineral-coated hydroxyapatite microparticle; TGF, transforming growth factor.