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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Microsc Microanal. 2015 Sep 7;21(5):1224–1235. doi: 10.1017/S1431927615015007

Figure 2.

Figure 2

Aligning multiple whole-slide images. Above: a conceptual diagram showing that the registration, i.e., generation and tracking of the global transformation, between two large whole-slide images enables synchronized visualization of the specimens at any chosen scale, rotation, and translation. Arrows show transformations being tracked by software. Below: a clinically relevant example of aligning two immunostained serial sections of lung fine needle aspirate specimens showing differential diagnosis of a prostate adenocarcinoma metastasized to lung. Arrow heads indicate where nuclear positivity of thyroid transcription factor 1 (TTF1) characterized lung epithelial cells. Arrows indicate that cytoplasmic staining of prostate-specific membrane antigen (PSMA) as well as the absence of TTF1 staining revealed prostate originality of cancer cells.