Effect of TP1 on the fibrinogenolytic activity of trypsin, chymotrypsin and elastase. SDS-PAGE (10%) under reducing conditions. Coomassie blue staining. Trypsin (Try, 0.05 nM), chymotrypsin (QTry, 0.05 nM), elastase (Elas, 0.1 nM). Fibrinogen (Fg, 25 μg) and TP1 (at a 1:4, 1:40 and 1:20 enzyme:inhibitor ratio, preincubated for 30 min at 37 °C). MW: protein molecular weight markers. Lanes: 1) Fg control; 2) Fg + Try; 3) Fg + (Try + TP1); 4) Fg + QTry; 5) Fg + (QTry + TP1); 6) Fg + Elas; 7) Fg + (Elas + TP1).