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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Cerebellum. 2016 Feb;15(1):14–17. doi: 10.1007/s12311-015-0712-6

Fig 1.

Fig 1

a Schematic illustrating the injection of WGA-Alexa 555 into the spinal cord of a pup. b WGA-Alexa 555 tracing of spinocerebellar mossy fibers (arrow) and transynaptic tracing of neuronal somata (double arrow) in the anterior cerebellum. c, d NFH staining revealed a pattern of zones that was complementary to a subset of traced Purkinje cells. e, Some of the WGA-Alexa 555 traced neuronal somata were co-labeled with NFH, a marker for Purkinje cells (yellow circles). The layers of the cerebellar cortex are indicated by ml (molecular layer), pcl (Purkinje cell layer), and gl (granular layer). The scale bar = 100 μm in B (applies to C, D) and 50 μm in E.