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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Immunogenetics. 2015 Dec 19;68(2):145–155. doi: 10.1007/s00251-015-0890-x

Fig. 1. Sequences of the altered DH alleles and the immunization strategy used.

Fig. 1

(A) Sequences of the single DH gene segments present in the D-altered strains. Germline DFL16.1 in the ΔD-DFL allele is shown at the top, frameshifted DFL16.1 in the ΔD-DμFS allele is shown in the middle, and inverted DSP2.2 replacing the middle sequences of DFL16.1 in the ΔD-iD allele is shown at the bottom. (B) Impact of DH germline sequence on the amino acid content of the CDR-H3 loop. The derivation of the most common amino acids at positions 101–103 is circled and compared to the sequence of the progenitor DH. Color code, charged amino acids are marked in red, hydrophobic amino acids are marked in blue, neutral amino acids are marked in green, and tyrosine is marked in black. (C) Immunization scheme for all studies described in this manuscript. The mice were primed at day 0 and boosted at the time points indicated by red arrows. Grey arrows indicate blood collection time points. N, non-immunized. P, prime. B1, B2, B3, and B4; boost #1, #2, #3 and #4, respectively. Details of the experimental protocol and immunogen formulations are provided in Materials and Methods.