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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Jul 27;35(15):1988–1995. doi: 10.1038/onc.2015.275

Figure 2. ARF does not interfere with Mule-Miz1 binding.

Figure 2

(A) HEK293 cells were transfected with M2-Mule, which was then immunoprecipitated and subjected to in vitro binding assay with purified His-Xpress-Miz1 in the presence of various concentrations of purified ARF. (B) Cells were transfected with control siRNA or siRNA for ARF, followed by treatment without or with TNF in presence of MG-132. The interaction between Mule and ARF was determined by immunoprecipitation with Mule antibody followed by immunoblotting with Miz1 antibody.