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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Jul 27;35(15):1988–1995. doi: 10.1038/onc.2015.275

Figure 3. TNF induces ARF-Mule dissociation.

Figure 3

(A) HEK293 cells were treated with TNF for 0, 2, and 5 min. The interaction between Mule and ARF was determined by immunoprecipitation with Mule antibody, followed by immunoblotting with p14ARF antibody. (B) In vitro ubiquitination of GST-Miz1 by immunoprecipitated endogenous Mule from HEK293 cells treated with TNF for various times as indicated. (C) In vivo ubiquitination of Miz1 in cells treated with TNF for various times as indicated.