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. 2016 Jan 27;11(1):e0148062. doi: 10.1371/journal.pone.0148062

Fig 4. MycN knockdown affects the expression of cell cycleand autonomic neuron development-related genes.

Fig 4

FACS-sorted p75+ hNCSCs were transduced with concentrated pGLVH1/GFP-MYCN shRNA virus and selected in puromycin for two weeks.(A): Real-time PCR analysis showing the expression of cell cycle-related genes was altered by MycN suppression, *p<0.05, ***p<0.001; ****p<0.0001; quantitative data was acquired from three independent experiments;(B): Western blot shows knockdown of MycN increases the expression of p15, whereas decreases the expression of Cyclin D1; (C) Real-time PCR analysis showing the expression of polycomb genes and autonomic neuronal markers was altered by MycN suppression, *p<0.05, **p<0.01;***p<0.001; ****p<0.0001, quantitative data were acquired from three independent experiments.