Skip to main content
. 2016 Jan 27;11(1):e0148062. doi: 10.1371/journal.pone.0148062

Fig 5. MycN knockdown suppresses cell cycle progression in hNCSCs.

Fig 5

FACS-sorted p75+ hNCSCs were transduced with concentrated pGLVH1/GFP-MYCN shRNA virus and selected in puromycin for two weeks.(A): Cell growth of control and MycNkd hNCSCs was detected by MTT assay, cell growth is presented as percentage increase: Absorbance dn–absorbance d0 / absorbance d0and error bars representing 95% confidence intervals are shown. Data are from three independent experiments, ***p<0.001; (B): Cell cycle was assessed in adherent control and MycNkd hNCSCs 2weeks after lentiviral transduction; (C): Flow cytometric of DNA content showed MycN kd hNCSCs was arrested in G0/G1, *p<0.05, **p<0.01.