Figure 1. Cartoon schematic of experimental design.
Standard mouse cages were modified to restrict access to the food chamber. Access was controlled by a motorized gate controlled by timed relay switches. Mice could only access food pellets when the gate was lifted (6 hr), and positive drive of the motor kept the gate closed for the remaining 18 hr. The scheduled feeding protocol was maintained for a minimum of 2 weeks prior to and during sample collections and behavioural tests, indicated by *. PER2::LUC, PER2-driven bioluminescence. LTP, long term potentiation. FC, fear conditioning. NOR, novel object recognition.