Table 1.

Summary of experimental procedures

Procedure	Number of Objects
Euthanize rats	(10 rats) × 3 days
Isolate inner medullas	(20 inner medullas) × 3 days
Make IMCD suspensions	(1 suspension) × 3 days
Divide suspension into two samples	(2 samples) × 3 days
Incubate one sample with dDAVP and one sample with vehicle	(1 dDAVP sample + 1 vehicle sample) × 3 days
Fractionate each sample to obtain NE and NP fractions	(1 dDAVP/NE sample + 1 dDAVP/NP sample +1 vehicle/NE sample + 1 vehicle/NP sample) × 3 days
Pool samples	1 dDAVP/NE sample + 1 dDAVP/NP sample +1 vehicle/NE sample + 1 vehicle/NP sample
Run SDS-PAGE for each pooled sample	4 half-gels
Slice gels	39 slices × (1 dDAVP/NE + 1 vehicle/NE) +36 slices × (1 dDAVP/NP + 1 vehicle/NP)
In gel trypsinization	150 samples
LC-MS/MS	150 samples

See text for details.