Figure 2.

Deep tumor detection and laser ablation with sub-millimeter precision. (A) Two pieces of tumor tissue were harvested from a mouse with primary STS six hours injection of LUM015. These tumor pieces were placed under tissue phantoms of different thicknesses and the fluorescence was measured through the phantom. Background fluorescence of the 10 mm phantom was also determined as a baseline measurement. (B, C) Three mice with primary sarcomas were injected with LUM015 6 hours prior to harvesting tumor tissues. Three tumor cubes were taken from each mouse (9 cubes total), visualized with the laser ablation system and subjected to one, two or three passes of the laser. Each ablated tumor cube was then formalin fixed and paraffin embedded (FFPE) and cut into 10 µm sections. (B) Representative hematoxylin and eosin (H&E) stained sections of resected tumor pieces from mice showing the ablation trench that resulted from one, two and three passes of the ablation laser. The trench is identified with arrows indicating its dimensions in µm (Scale bar = 200). (C) A box plot of the ablation trench depth as a function of laser passes as measured on separate H&E sections, showing that the depth of ablation can be controlled with micron-level precision, depending on the number of passes (One-way ANOVA, P<0.0001).