Figure 2.

Optimization of liposome composition and in vitro efficacy of siKSP@SLNPs in PC-3 cells. (A, B) KSP gene silencing by SLNPs formed at various molar ratios of MA-Chol to DOPE and mol% PEG₁₀₀₀-DSPE in the absence (A) or presence (B) of serum. KSP mRNA levels were evaluated to determine the optimal molar ratio of MA-Chol to DOPE (1:1 or 1:2) at varying PEG₁₀₀₀-DSPE concentrations (1, 2.5, and 5 mol%). Results are shown as mean ± s.e. (n=6). (C) Cells were transfected for 4 h with siKSP@SLNPs comprised of MA-Chol/DOPE (1:1) and cytotoxicity was analyzed 48 h later. siKSP@SLNPs exhibited significantly greater cytotoxicity than control formulations. Data represent mean ± s.e. (n=9). ***p < 0.001. (D) Western blot analysis following that transfection of cells with siKSP@SLNPs resulted in substantial knockdown of KSP protein expression in PC-3 cells. Results in duplicate samples are shown.