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. 2016 Jan 1;6(2):192–203. doi: 10.7150/thno.13657

Figure 6.

Figure 6

Biodistribution and in vivo antitumor activity of siKSP@SLNPs. (A) In vivo fluorescence imaging of PC-3 tumor-bearing nude mice after a single intravenous injection of 30 µg of Cy5.5-labeled free siRNA or an equivalent amount of Cy5.5-siRNA@SLNPs. (B) Ex vivo fluorescence imaging of the tumor and major vital organs (liver, heart, kidney, spleen, and lung) harvested from euthanized mice 24 h after injection. (C) Antitumor activity of low- and high-dose siKSP@SLNPs (0.5 and 1 mg/kg, respectively) in PC-3 tumor-bearing nude mice (n=5 mice/ group). *p < 0.05, **p < 0.01. (D) TUNEL assays to detect apoptotic cells in tumor sections. Tumor tissues were collected 24 h after final administration. (E) KSP mRNA levels in tumor tissues were evaluated by qRT-PCR and normalized relative to GAPDH mRNA levels in the same samples (*p < 0.05, ***p < 0.001). (F) KSP protein expression in tumor tissues was analyzed by western blotting.