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. 2016 Jan 8;7:10002. doi: 10.1038/ncomms10002

Figure 3. CSF-c neurons are sensitive to pH changes.

Figure 3

(a) Whole-cell patch recording of a CSF-c neuron, showing mechanosensitivity to a fluid-pulse (20 p.s.i., 80 ms). (b) The same neuron firing spontaneous action potentials in control conditions (pH 7.4) in the presence of gabazine (20 μM), AP5 (100 μM) and NBQX (40 μM). (c–e) Lowering pH to 6.9 increased action potential frequency (c; red), and more so at pH 6.5 (e; orange) Also, the membrane potential was depolarized by ∼5 mV at lowered pH. (f) Action potential frequency during 1 min in CSF-c neurons at pH 7.4, 6.9 and 6.5, respectively. The values are means±s.e.m., normalized to basal activity at pH 7.4 (n=15). Student's t-test: ***P<0.001, significant difference compared with control, pH 7.4. (g) Cell-attached patch recording of a CSF-c neuron showing the response to fluid-pulse stimulation. (h–j) This mechanosensitive CSF-c neuron in addition responded by increased action potential frequency to lowered pH (6.9), also evident in the cell-attached recording configuration. (k–m) In addition, subthreshold depolarizing receptor potentials appeared with lowered pH (pH 6.9).