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. 2016 Jan 8;7:10002. doi: 10.1038/ncomms10002

Figure 4. APETx2 inhibits chemosensitivity of CSF-c neurons.

Figure 4

(a) CSF-c neuron showing mechanosensitivity to a fluid pressure pulse (80 ms, 20 p.s.i.). (b,c) In the same neuron, the action potential frequency increased at pH 6.9 in the presence of gabazine (20 μM), AP5 (100 μM) and NBQX (40 μM). (d) Application of APETx2 (2 μM) abolished the response to fluid-pulse stimulation. (e,f) APETx2 also abolished the response to pH lowering. (g) Firing of CSF-c neurons before and after application of APETx2. The values are means±s.e.m. during 1 min of recording, normalized to basal activity at pH 7.4 (n=5). Student's t-test: ***P<0.001, significant difference at pH 6.9 compared with 7.4 only in control conditions in the absence of APETx2. (h) Decreases in pH resulted in depolarizing potentials also in the presence of TTX (1.5 μM), that reversed at positive holding potentials. (i) Addition of APETx2 (1 μM) completely blocked the response to lowered pH in the presence of TTX (1.5 μM).