Figure 1. ESL-1 controls the proliferation and numbers of HSPC in the bone marrow.

(a) Representative plots of BrdU proliferation assays gated on Lineage^NEG cKit^HI Sca1⁺ (LSK) cells, LSK CD48^NEG and LSK CD48^NEG CD150⁺ cells in Glg1^−/− and Glg1^WT/− littermates. Right, percentage of BrdU-incorporating cells; n=4. (b) Percentage of the indicated populations in the BM of Glg1^−/− and Glg1^WT/− littermates; n=5. (c) Experimental design and percentage of BrdU incorporation in WT, Glg1^−/−and Selplg1^−/− progenitors in transplanted WT mice; n=9–11. (d) Percentage of BrdU-incorporating cells in the indicated HSPC populations from WT and Glg1^−/−-transplanted mice; n=4. (e) Absolute numbers of the indicated populations in two femurs of WT and Glg1^−/− transplanted mice; n=7. (f) Survival curves of mice transplanted with WT or Glg1^−/− BM cells after repeated injection of 5-FU; n=10. *P<0.05; **P<0.01; ***P<0.005 as determined by Student's t-test for two data sets, or one-way analysis of variance with Turkey's test for multiple data sets. In f, comparison of survivals was performed using the Log-rank test. Each circle represents a mouse. Bars show mean±s.e.m.