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. 2016 Jan 8;7:10255. doi: 10.1038/ncomms10255

Figure 2. Rescue of free Raptor prevents aging- and obesity-dependent hepatic steatosis.

Figure 2

(a) Western blot from livers of adult Ad-GFP and Ad-Raptor male mice, following size-exclusion chromatography. Fractions 24 and 25 correspond to mTORC1-associated (∼800 kDa) Raptor, while 34 and 35 to free (∼150 kDa) Raptor. (b–d) Hepatic triglyceride (TG) in young or adult (n=6/group) (b), aged (10- to 12-month-old) (n=7 per group) (c), or DIO (n=5 per group) (d) Ad-GFP and Ad-Raptor male mice. (e–g) Plasma TG (e), hepatic fatty acid synthesis (f) and lipogenic gene expression (g) in young or adult, Ad-GFP and Ad-Raptor mice, killed after a 16 h fast followed by 4 h refeeding (n=6 per group). *P<0.05, **P<0.01 as compared with the indicated control by two-way analysis of variance. All data are shown as the means±s.e.m. All blots are representative of three independent experiments, and samples within each group chosen randomly.