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. 2016 Jan 8;7:10268. doi: 10.1038/ncomms10268

Figure 3. Food intake studies with GsD-AgRP, hM3Dq-AgRP and WT control mice.

Figure 3

CNO-mediated activation of GsD in AgRP neurons promotes food intake both acutely (a) and over a 3-day period (b), and increases body weight (BW) (c). Measurements were carried after a single i.c.v. injection of either saline or CNO (1 μg) into GsD-AgRP mice. CNO-induced activation of hM3Dq in AgRP neurons stimulates food intake acutely (d) but not chronically (e). BW was increased only on day 1 (f). Measurements were carried after a single i.c.v. injection of either saline or CNO (1 μg) into hM3Dq-AgRP mice. (g–i) Treatment of WT control mice with an active AgRP fragment (AgRP83–132) promotes food intake acutely (g) and over a 3-day period (h), and leads to increased BW (i). Measurements were carried out after a single i.c.v. injection of either saline or AgRP83–132 (3 μg). Injections were carried out between 9:00 and 10:00. All experiments were carried out with 12- to 16-week-old male mice. Data are given as means±s.e.m. (n=4–7 mice per group). *P<0.05, **P<0.01, ***P<0.001, as compared with the corresponding saline-injected group (Student's t-test).