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Identification of Ag85A expression by indirect ELISA. An ELISA plate coated with equal amounts of BCG and rBCG::Ag85A bacteria was probed for Ag85A expression with serially diluted mAb 6B5. Expression of Ag85A by rBCG::Ag85A was significantly higher than that by BCG. Data depicted are the mean values ± SEM. Statistical significance was determined by a Student's t-test.
