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. 2016 Jan 28;6:3. doi: 10.3389/fcimb.2016.00003

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Copyright © 2016 Xu, Chen, Hu, Meng, Wang, Rao, Zhang, Yin, Pan and Jiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or repr oduction is permitted which does not comply with these terms.

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Ex vivo detection of antigen-presentation following rBCG::Ag85A infection. After s.c. administration of rBCG::Ag85A, DCs from mouse lymph nodes were analyzed for their ability to stimulate the DE10 T hybridoma by probing for Ag85A peptide presentation in MHC complexes. Ag85A-M was detected 4 h post-rBCG infection. rBCG::Ag85A induced higher levels of Ag85A-specific antigen-presenting activity in mice compared with those immunized with BCG. Data depicted are the mean values ± SEM. Statistical significance was determined by a Student's t-test (^*P < 0.05, ^**P < 0.01).