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. 2016 Jan 28;6:1233. doi: 10.3389/fpls.2015.01233

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Copyright © 2016 Docimo, Francese, Ruggiero, Batelli, De Palma, Bassolino, Toppino, Rotino, Mennella and Tucci.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SmMYB1 interacts with bHLH TF StbHLH1 in the yeast two-hybrid assay. SmMYB1 and SmMyb1Δ9 were cloned in the prey plasmid pGADT7 and co-transformed with StbHLH1pGBKT7 (D’Amelia et al., 2014) in yeast strain AH109. The SmMyb1pGADT7/pGBKT7 and pGADT7/StbHLH1pGBKT7 combinations served as negative controls while StbHLH1/StAN1 is shown as positive control. Yeast cells grown on synthetic complete media (–W/–L, Right) and on selective media (–W/–L/–H/–A, Left) are shown. Pictures were taken 3 days after incubation at 30°C.