Table 3.

Functional annotations (SEED subsystem level I) that are overrepresented in microbialites relative to the surrounding environment by ANOVA using STAMP.

Seed subsystem	p-val (corr)	Effect Size	Fil: Avg Rfeq (%)	Fil: SD (%)	MB: Avg Rfeq (%)	MB: SD (%)	Sed: Avg Rfeq (%)	Sed: SD (%)
Membrane transport	8.12E-03	0.793	1.998	0.157	2.514	0.112	0.941	0.577
Metabolism of aromatics	3.00E-04	0.934	1.27	0.074	1.775	0.111	0.872	0.109
Motility and chemotaxis	3.60E-03	0.649	0.606	0.025	1.063	0.039	0.454	0.356
Potassium metabolism	6.40E-03	0.81	0.182	0.035	0.296	0.013	0.084	0.068
Regulation and cell signaling	1.82E-03	0.874	1.09	0.028	1.506	0.079	0.978	0.133
Virulence, disease, and defense	3.45E-04	0.928	2.069	0.195	2.997	0.057	1.167	0.327

STAMP ANOVA tested significant differences between multiple groups (microbialite, sediments, and filters) using a post hoc test (Tukey–Kramer at 0.95), an effect size (Eta-squared), a p-value (<0.01) and a multiple-test correction of Benjamini–Hochberg FDR. Results are for RefSeq classifications that significantly differed (p < 0.01) for microbialite over the surrounding environments (sediments and filters). Avg Rfreq, Average relative frequency; SD, Standard deviation; p-val corr, p-value ANOVA corrected; MB, Microbialite; Sed, Sediment.

Avg Rfreq, Average relative frequency; SD, Standard deviation.

p-val corr, p-value ANOVA corrected (Benjamini-Hochberg FDR).

Fil, Filter represents surrounding water; MB, Microbialite. Sed: Sediment.