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. 2016 Jan 12;7:10230. doi: 10.1038/ncomms10230

Table 2. Primers used for RT-qPCR analysis.

Genes Species Forward primer Reverse primer [C]
CCL7 Murine 5′- AAGATCCCCAAGAGGAATCTCAA -3′ 5′- CTTCCCAGGGACACCGACTA -3′ 900 nM
CCL7 Human 5′- AAACCTCCAATTCTCATGTGGAA -3′ 5′- CAGAAGTGCTGCAGAGGCTTT -3′ 900 nM
Leptin Murine 5′- GGGCTTCACCCCATTCTGA -3′ 5′- TGGCTATCTGCAGCACATTTTG -3′ 900 nM
Leptin Human 5′- CCTTCCAGAAACGTGATCCAA -3′ 5′- GGCCAGCACGTGAAGAAGAT -3′ 900 nM
Adiponectin Murine 5′- TGGAATGACAGGAGCTGAAGG -3′ 5′- TATAAGCGGCTTCTCCAGGCT -3′ 300 nM
Adiponectin Human 5′- GCAGAGATGGCACCCCTG 5′- GGTTTCACCGATGTCTCCCTTA -3′ 900 nM
TNF-α Murine 5′- TGGGACAGTGACCTGGACTGT -3′ 5′- TTCGGAAAGCCCATTTGAGT -3′ 300 nM
TNF-α Human 5′- CCGAGTCTGGGCAGGTCTAC -3′ 5′- TGGGAAGGTTGGATGTTCGT -3′ 300 nM
GAPDH Murine and Human 5′- TGCACCACCAACTGCTTAGC -3′ 5′- GGCATGGACTGTGGTCATGAG -3′ 500 nM
HPRT Murine and Human 5′- TGGCCATCTGCCTAGTAAAGC -3′ 5′- GGACGCAGCAACTGACATTTC -3′ 500 nM

RT-qPCR, quantitative reverse transcription PCR.