Table 2. Primers used for RT-qPCR analysis.
Genes | Species | Forward primer | Reverse primer | [C] |
---|---|---|---|---|
CCL7 | Murine | 5′- AAGATCCCCAAGAGGAATCTCAA -3′ | 5′- CTTCCCAGGGACACCGACTA -3′ | 900 nM |
CCL7 | Human | 5′- AAACCTCCAATTCTCATGTGGAA -3′ | 5′- CAGAAGTGCTGCAGAGGCTTT -3′ | 900 nM |
Leptin | Murine | 5′- GGGCTTCACCCCATTCTGA -3′ | 5′- TGGCTATCTGCAGCACATTTTG -3′ | 900 nM |
Leptin | Human | 5′- CCTTCCAGAAACGTGATCCAA -3′ | 5′- GGCCAGCACGTGAAGAAGAT -3′ | 900 nM |
Adiponectin | Murine | 5′- TGGAATGACAGGAGCTGAAGG -3′ | 5′- TATAAGCGGCTTCTCCAGGCT -3′ | 300 nM |
Adiponectin | Human | 5′- GCAGAGATGGCACCCCTG | 5′- GGTTTCACCGATGTCTCCCTTA -3′ | 900 nM |
TNF-α | Murine | 5′- TGGGACAGTGACCTGGACTGT -3′ | 5′- TTCGGAAAGCCCATTTGAGT -3′ | 300 nM |
TNF-α | Human | 5′- CCGAGTCTGGGCAGGTCTAC -3′ | 5′- TGGGAAGGTTGGATGTTCGT -3′ | 300 nM |
GAPDH | Murine and Human | 5′- TGCACCACCAACTGCTTAGC -3′ | 5′- GGCATGGACTGTGGTCATGAG -3′ | 500 nM |
HPRT | Murine and Human | 5′- TGGCCATCTGCCTAGTAAAGC -3′ | 5′- GGACGCAGCAACTGACATTTC -3′ | 500 nM |
RT-qPCR, quantitative reverse transcription PCR.