FIGURE 5.

Formation of amyloid inclusions in E. coli cells expressing MccE492 variants with altered aggregation propensity. p33pETAB constructs expressing different variants of MccE492 were transformed into E. coli BL21-AI cells. After 6 h of induction, cells were fixed with PFA, stained with either BTA-1 or ThS and visualized by confocal microscopy. Scale bar: 5 μm.