FIGURE 6.

In vitro amyloid polymerization of MccE492 variants with altered aggregation propensity. MccE492 variants (0.4 mg/ml) were incubated in aggregation buffer (see Materials and Methods) and the polymerization progress was evaluated during the time using Congo red binding (A), and visualizing the remaining soluble MccE492 (after centrifuging the samples at 16000 × g during 40 min) through SDS-PAGE- Immunoblot (B). In addition, the antibacterial activity was determined at each time (C), and the morphology of the fibrils was studied by negative-stain electron microscopy (D). Error bars show the standard deviation from three independent experiments. Scale bar: 100 nm.