FIGURE 2.

Accumulation of FLAG multimers in seeds of two different tobacco varieties. (A) Analysis of ripe T₃ seeds of N. tabacum cv. SNN USP-FIC line 28/10 and T₂ seeds of N. tabacum cv. Petit Havana USP-FIC line 49 by gradient SDS-PAGE (4–10% PAA) with 20 μg total soluble seed protein per lane, Western blotting and immunodetection based on the c-myc tag; kDa, kilodalton. (B) Transgenic USP-FIC line 49 with a genomic background of N. tabacum cv. Petit Havana as well as the corresponding wild type plants showed a faster vegetative growth and flowered 8 days earlier than Samsun NN-genome-based FLAG overexpressing plants of USP-FIC line 28/10 and its corresponding wild type plants.