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. 2016 Jan 28;6:324. doi: 10.3389/fphar.2015.00324

FIGURE 4.

FIGURE 4

Validation of binding interaction between miRNA–mRNA by RNA electrophoretic mobility shift assays (RNA EMSAs): (A) miR107–MYB; (B) miR10a–GALNT1 and (C) miR378–GZMB. RNA EMSAs were performed using cy5-labeled respective miRNA oligonucleotide and 2′-O-methyl modified and IRD-800 labeled mRNA oligonucleotides. Lanes 1 and 2 show the mobility of the respective labeled mRNA or miRNA oligonucleotide. Lane 3 shows the mobility of the labeled miRNA oligonucleotide with its target mRNA oligonucleotide. Lanes 4 and 6 show the mobility of labeled mRNA oligonucleotide in presence of excess unlabeled specific competitor miRNA oligonucleotide and excess unlabeled non-specific competitor (NC). Lane 5 shows the mobility of labeled mRNA oligonucleotide in presence of excess unlabeled specific competitor mRNA oligonucleotide.