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. 2016 Jan 28;7:7. doi: 10.3389/fmicb.2016.00007

FIGURE 1.

FIGURE 1

TspR is required for the expression of T3SS genes. (A) The expression of exoS-lux was measured in the wild-type PAO1, tspR::Gm, and the complemented tspR::Gm strain (tspR::Gm/p-tspR), the tspR transposon strain (tspR::Tn), and the tspR::Tn complemented strain (tspR::Tn/p-tspR). The asterisks indicate the statistically different exoS expression compared to that in the wild-type strain as determined by Student’s t-test (∗∗∗P < 0.001). (B) The expression and secretion of ExoS were examined in the indicated strains by Western-blotting. Strains were grown in LB with EGTA to an OD600 = 0.6. Whole-cell extracts from the designated strains were separated by SDS-PAGE and immuno-blotted. (C) Effect of tspR mutation on swarming motility. Overnight cultures were spotted onto swarming plates (2 μl aliquots) and the plates were incubated at 37°C. The images captured after 16 h of growth. The experiments were repeated at least three times and similar results were observed. (D) TspR is required for biofilm formation. Quantification of crystal violet (CV) staining of biofilms was grown in microtiter plates for 14 h. ∗∗P < 0.005 based on Student’s t-test. Photo of the tube binding assay was taken. EV, empty vector.