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. 2016 Jan 28;7:37. doi: 10.3389/fmicb.2016.00037

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Copyright © 2016 Loughman, Hall, Knowlton, Sindeldecker, Gilson, Schmitt, Birch, Gajtka, Kobe, Florjanczyk, Ingram, Bakshi and Horzempa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Francisella tularensisΔpyrF is not more resistant to Gm than wild type LVS. Bacteria were cultivated in general growth media, suspended in TSBc, and treated with Gm at the indicated temperature. Bacteria were diluted and plated for viable CFU at the indicated times. Plotted values represent mean CFU ± SD. Log-transformed data were analyzed using a two-way ANOVA and a Sidak’s multiple comparisons test. LVS 26°C vs. ΔpyrF Gm (100 μg/ml) not significant. ^∗P < 0.05 LVS 26°C vs. 37°C Gm (100 μg/ml) at 1 and 2 h; ^∗P < 0.05 LVS 37°C vs. ΔpyrF Gm (100 μg/ml) at 1 and 2 h.