Figure 6. Atg1^PC tethers Atg9–PLs.

(a) The hydrodynamic radius of SUVs (white bar) and Atg9–PLs (black bar) obtained from DLS-experiments in the absence or presence of Atg17 and corresponding variants as illustrated by cartoons are shown. ND, not determinable (polydisperse). (b) Similar experiments shown in a were performed for Atg17 and Atg17-variants and that of corresponding Atg17^TC and Atg1^PC (sub)complexes. (a,b). Mean values±s.d. of N=5 experiments are shown for both a and b. P values were calculated using two-tailed Student's T-test (*P<0.05; **P<0.01; ***P<0.001). (c) The schematic drawing illustrates the experimental setup and summarizes the results obtained from DLS-experiments in a and b. (d) Cyro-electron micrographs of Atg9–PLs from samples used for DLS-experiments containing Atg17, Atg17^TC, Atg17^mono, and Atg1^PC. Atg17 tethers two and Atg1^PC tethers multiple vesicles together. Scale bar, 50 nm.