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. 2016 Jan 12;7:10329. doi: 10.1038/ncomms10329

Figure 4. Yap is required for proliferation and polarity of ependymalprogenitor cells.

Figure 4

(a) Yap is highly expressed in nucleus, cytoplasm and apical surface (enlarged picture) of progenitor cells, but is no longer detectable in the Yap CKO. (b,c) Compared with WT, Yap CKO shows fewer proliferating cells marked by BrdU incorporated by S-phase precursors at E14.5. (d–g) Yap CKO aqueduct contains fewer Sox9 (+) cells than WT at E14.5 (arrows). The fraction of cells exiting cell cycle (Sox9 (−) BrdU (+)/total BrdU (+) cells 24 h after BrdU labelling) is increased in the Yap CKO compared with WT. (h) βIII-tubulin (+) processes cover the ventricular surface of Yap CKO, whereas VZ is largely devoid of βIII-tubulin (+) processes in the WT. In the Yap CKO, NeuN (+) cells are also shifted to the VZ where progenitor cells are scarce. (i) adherens junction protein, N-Cadherin, which is concentrated in the apical endfeet in WT, are diminished in the Yap CKO (arrows). Apical polarity complex protein Crb, detected by pan-Crb protein antibody, has largely disappeared from the apical surface of the aqueduct in the Yap CKO (arrows). Quantification of BrdU (+) and Sox9 (+) cells was done by counting positive cells in the images, excluding SCO (n=3). Two-tailed unpaired t-test reveals statistical significance (**P<0.01 and *P<0.05). Error bars represent mean+s.e.m.; n=3. Scale bars, 50 and 20 μm (a); 50 μm (b); 100 and 20 μm (d,h,i).