Figure 3.

Effects of spermine on HG-induced cytochrome C release, bcl-2 decrease, and caspases activation in primary cardiomyocytes. A. Protein levels of cyto-Cyt C, mt-Cyt C in the NG, NG+SP, HG, HG+SP groups, as evaluated by western blot assay. GAPDH was used as a loading control for total cell lysate. Hsp60 was used as a loading for mitochondria (mt) protein. B. Protein levels of bcl-2 and cleaved caspase-3/9 in the four groups assessed by western blot assay. Quantification of cyto-Cyt C, mt-Cyt C, bcl-2, and cleaved caspase-3/9 expression compared with control protein. Data are presented as means ± SEM; n = 3 per group; *P<0.05 vs. control group (NG or HG group); **P<0.01 vs. control group; ***P<0.001 vs. control group.