Figure 4.

Effects of spermine on T-SOD, MDA, GSH, GSSG changes and ROS generation in HG-treated primary cardiomyocytes. A. T-SOD activity; B. MDA content; C. GSH/T-GSH ratio; D. GSSG/T-GSH ratio. All parameters were detected by the corresponding kits. E. DCFH-DA staining followed by photofluorography was carried out to observe intracellular ROS levels. The mean fluorescence intensity (MFI) of DCFH-DA staining of four groups in right panel was quantitative analysis with Image-Pro Plus software. Data are presented as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001 vs. control group (NG or HG group).