Figure 6.

Examine the protective effect of spermine through comparing with NAC (a well-known antioxidant). A. the anti-apoptotic effect comparison with spermine and NAC. Cells were pretreated as described, and then the protein expression of cleaved caspase3 was examined. B. the antioxidantive effect comparison with spermine and NAC. Cells were pretreated as described, and then intracellular ROS level were analyzed. C. spermine inhibited the activation of p38/JNK and JAK2 pathways to decrease ROS generation, not only scavenged ROS to suppress p38/JNK and JAK2 pathways as NAC. Data are presented as the means ± SEM; n = 3 per group; *P<0.05, **P<0.01. control group (HG or HG+NAC group).