Figure 3.
Effect of miR-30a-5p on caspase-3/7 activity in HCC cell lines. HepG2 (A), SMMC-7211 (B), HepB3 (C) and SNU449 (D) cells (2.5 × 103 cells per well in 96-well-plate) were treated as aforementioned in Figure 1. After the cell viability was monitored with fluorimetric detection of resorufin, caspase-3/7 activity (Apo-ONE Homogeneous Caspase-3/7 Assay) was detected with the same samples. Columns were the average of three independent experiments. Bars represented standard deviation. *P < 0.05, **P < 0.01 and ***P < 0.001, compared to blank and negative controls at the same time point.