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Hematoxylin and eosin staining of the proximal metaphysis of the tibia, trabecular bone zone below growth plate was shown (A: 50×). Bone volume over total volume (BV/TV), trabecula number (Tb. N), trabecula thickness (Tb. Th) and trabecula separation (Tb. Sp) were measured (B). Values were expressed as mean ± SD, n = 6 in each group. *P < 0.05 versus vehicle group, #P < 0.05 versus GIOP group.
