Figure 2.

VEGF-D expression was correlated with CCR7 in different NSCLC cell lines and reduced by CCR7 antibody. The correlation between the VEGF-D and CCR7 in mRNA (A) and protein (B) levels in NSCLC cell lines. Cells were cultured in the same condition and analysis the protein and mRNA by Western blot and RT-PCR. (C) CCR7 antibody reduced the expression of VEGF-D protein in A549 cells. A549 cells were serum-starved and treated with CCR7 antibody for indicated times. (D) A549 cells were transfected with scrambled siRNA sequence (Sc) or siRNA for CCR7 (Si) followed by incubation for 48 h. Total protein was isolated and expression of CCR7 and VEGF-D was analyzed by Western blot.