Figure 3.
CCR7 induced ERK1/2 and Akt phosphorylation in lung cancer cells. The expression of p-ERK1/2, ERK1/2, p-Akt, Akt protein were determined by western blotting. A. The expressions of p-ERK1/2 and p-Akt were increased in BE1-CCR7 cells, while exhibiting no effect on the total protein levels of ERK1/2 or Akt. B. A549 cells were transfected with scrambled siRNA sequence (Sc) or siRNA for CCR7 (Si) followed by incubation for 48 h. Total protein was isolated and expression of phosphorylation of ERK1/2 or Akt was determined by western blot analysis.