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Effect of silencer RNA for Sirt1 on inhibition of acute lung injury by rubriflordilactone inMLE-15 cells. The cells transfected with siRNA for Sirt1 were exposed to LPS after rubriflordilactone treatment and then analyzed by western blot analysis. Rub, LPS, Sirt1, MMP-9, IL and iNOS stand for rubriflordilactone, lipopolysaccharide, sirtuin 1, matrix metalloproteinase-9, interleukin and inducible nitric oxide synthase.
