Figure 2. Splenocyte-secreted cytokines upon DENV1-4 antigen stimulation were detected by ELISPOT (n = 5).
(A) The numbers of cytokine-positive cells are expressed as spot-forming units (SFU)/5 × 105 cells after background subtraction. (a) DENV1 antigen stimulation (*p < 0.05, **p < 0.01). (b) DENV2 antigen stimulation (*p < 0.05, **p < 0.01). (c) DENV3 antigen stimulation (*p < 0.05, **p < 0.01). (d) DENV4 antigen stimulation (*p < 0.05, **p < 0.01). (B) a representative raw ELISPOT data in the format of scanned image, a–d: stimulated by DENV1-4 antigens, respectively.