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. 2016 Jan 28;6:18643. doi: 10.1038/srep18643

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Copyright © 2016, Macmillan Publishers Limited

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(A–D) Quantitative RT-PCR was used to analysis expression levels of PtoCAD1, PtoCCR2, PtoC4H2 and PtoSND1-A1, respectively. (E) PtrWRKY19 binds to the PtoC4H2 promoter. Schematic of the PtoC4H2-GUS reporter (top) and PtrWRKY19::GFP effectors (bottom). Transcription level of the PtoC4H2 promoter reporter by PtrWRKY19 was assessed by GUS activity. PtoC4H2-GFP was used as a control. Error bars represent ±SD from 3 independent experiments. **P < 0.01, Student’s t test.