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(A) Coomassie stained gel showing equal amounts of pure GST-tag removed preparations of SEPT7 WT and mutants. (B) Native guanine nucleotide association of recombinant SEPT7 GTPase domains monitored by mass spectrometry. (C) Regenerative coupled-enzyme GTP hydrolysis assays for SEPT7 wild-type (WT), G59V, S63N, T89A and E202A mutants.
