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. 2016 Jan 28;6:20007. doi: 10.1038/srep20007

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(A) Rescue clones obtained by single-cell cloning strategy with double transduced MEFs. Indicated numbers of wells were seeded in 96-well plates in the presence or absence of doxycycline and the number of wells which successfully gave actively proliferating cell clones are indicated. (B–D) Verification of SEPT7 rescue clones. Genotyping analysis of the rescue clones (B), Immunoblot analysis for dox-inducible SEPT7 expression in the rescue clones with additional loading control blots (C) and SYTOX green staining for nuclei and microscopic anaylsis of multinucleation in the rescue clones propagated in the presence and absence of doxycycline (D). (E) Doubling time for the clones were calculated by using WST-1 cell proliferation assay and the results are tabulated.