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. 2016 Jan 28;6:19862. doi: 10.1038/srep19862

Figure 9. HpaR1 enhances RNA polymerase (RNAP) binding to the gumB promoter, as revealed by an electrophoretic mobility shift assay.

Figure 9

The gel shift assay was carried out using 1.0 nM of 529-bp FAM-labelled DNA probe of the gumB promoter. RNAP or/and HpaR1was/were added as follows: lanes 1, 2, 3 and 4 with 0, 0.05, 0.1 and 0.2 U of RNAP without HpaR1; lanes 5, 6 and 7 with 12.5, 15 and 17.5 nM of HpaR1 without RNAP; lanes 8, 9 and 10 with 12.5, 15 and 17.5 nM of HpaR1 and 0.05 U of RNAP; lanes 11, 12 and 13 with 0.05, 0.1 and 0.2 U of RNAP and 12.5 nM of HpaR1.