Table 1. Correlation between crystal system and full-length, long- and short-form of N-D1 truncates of p97.
| p97 construct | Residue range (in structure) | Nucleotide D1/D2 | Space group (No.) | PDB | No. sub/AU | Cell dimension (a, b, c, α, β, γ) | Res (Å) |
|---|---|---|---|---|---|---|---|
| WT FLp97 | 1–806 (21–763) | ADP/ADP-AlFx | I222 (23) | 3CF13 | 3 | 162.7 178.0 321.1 90.0 90.0 90.0 | 4.40 |
| WT FLp97 | 1–806 (21–763) | ADP/ADP | I222 (23) | 3CF33 | 3 | 164.0 178.9 320.6 90.0 90.0 90.0 | 4.25 |
| WT FLp97 | 1–806 (21–759) | ADP/AMP-PNP | P3 (143) | 3CF23 | 4 | 144.9 144.9 164.4 90.0 90.0 120.0 | 3.50 |
| WT FLp97a | 1–806 (17–735) | ADP/– | P622 (177) | 1R7R4 | 1 | 145.2 145.2 167.4 90.0 90.0 120.0 | 3.60 |
| WT ND1p97Shrt | 1–458 (21–458) | ADP/ | P622 (177) | 1E3210 | 1 | 146.0 146.0 84.7 90.0 90.0 120.0 | 2.90 |
| L198W ND1p97Shrt | 1–460 (15–460) | ADP/ | P622 (177) | 5DYGb | 1 | 145.6 145.6 84.0 90.0 90.0 120.0 | 2.20 |
| WT ND1p97Lng | 1–481 (21–461) | ADP/ | P212121 (19) | 5DYIb | 12 | 146.5 176.3 257.0 90.0 90.0 90.0 | 3.71 |
| R155H ND1p97Lng | 1–481 (18–462) | ADP/ | P212121 (19) | 4KOD6 | 12 | 146.5 170.7 256.6 90.0 90.0 90.0 | 2.96 |
| R86A ND1p97Lng | 1–481 (12–463) | ATPγS/ | P1 (1) | 3HU25 | 6 | 90.9 102.6 107.2 97.5 90.6 91.5 | 2.85 |
| R95G ND1p97Lng | 1–481 (12–462) | ATPγS/ | P1 (1) | 3HU15 | 6 | 92.8 103.3 107.7 97.7 91.9 89.8 | 2.81 |
| A232E ND1p97Lng | 1–481 (12–460) | ATPγS/ | P1 (1) | 4KLN6 | 6 | 91.2 104.5 109.5 98.1 90.6 92.7 | 2.62 |
| R155H ND1p97Lng | 1–481 (14–469) | ATPγS/ | H3 (146) | 4KO86 | 2 | 134.2 134.2 182.9 90.0 90.0 120.0 | 1.98 |
aReflection file is not available in PDB.
bStructures determined in this work.