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. 2015 Dec 22;17(1):4. doi: 10.3390/ijms17010004

Figure 2.

Figure 2

Expression, purification, Western Blot and pepsin digestion analysis of recombinant SaCSD1 protein. (a) Electrophoretogram of SaCSD1 protein. Lane M, protein marker; lane 1, uninduced E. coli lysate; lane 2, IPTG-induced E. coli lysate; lane 3, supernatant of the lysis after ultrasonication; lane 4, pellets of the lysis after ultrasonication; lane 5, the purified SaCSD1; (b) Western Blot detection of fusion protein with anti-His mAb. Lane 1, uninduced E. coli lysate; lane 2, the purified SaCSD1; (c) In vitro standardized pepsin digestion assay of SaCSD1 protein. Lane 1, the reaction without SaCSD1; lane 2, the reaction without pepsin; lane 3–8, the reaction in different times (0.5, 2, 5, 10, 20, 30 min); M, protein marker.