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FGF21 expression in the FGF21 stable monoclone (FM) cells before cell differentiation. (A) qRT-PCR analysis revealed that FM cells had higher FGF21 mRNA level than that in the control; and (B) Western blot analysis showed that the FGF21 protein level was up-regulated significantly in FM cells. Gene expression was normalized to GAPDH. Data was presented as mean ± SD, *** for p < 0.001.
