Figure 5.

hAgo2-mediated cleavage of target RNA with guide strands carrying either a 5’-uridine or a 5’-guanosine with or without a 5′-phosphate. Cleavage assays were conducted using 2.5 µM hAgo2, 100 nM guide RNA (as2b or G-as2b) and 2.5 nM target RNA (ICAM-1-IVT). Samples were taken at time points 0ʹ, 5ʹ, 25ʹ, 55ʹ, and 120ʹ and analyzed using 8% denaturing PAGE. Detection was carried out via autoradiography.